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β- Lactamase test reagent (id1810) cefnitrothiophene paper lactamase
Number of views:
1000

β- Lactamase test reagent (id1810) cefnitrothiophene paper lactamase

β- Lactamase test Purpose: Used to detect some bacteria β- Lactamases, such as Staphylococcus aureus, Haemophilus influenzae, Neisseria gonorrhoeae, Moraxella catarrhalis, Enterococcus faecalis and Bacteroides fragilis. Principle: The paper contains cefnitrothiophene. When this β The acyl bond in the lactam ring is β- After lactamase hydrolysis, a rapid color reaction occurs, changing from yellow to red, when a bacterium produces enough β Lactamase, apply the bacteria to the paper, and the paper will change from yellow to red. Cefnitrothiophene has a wide range of sensitivity and specificity. As a commercialized test β- So far, it has not been found that cefnitrothiophene will react with other microbial enzymes. β- Test method for lactamase one Hold the top of the test strip, slightly wet the filter paper in the test area at the end, and then directly dip or scrape the suspicious typical colonies with the filter paper wet at the end; two After incubation at 35 ℃± 2 ℃, observe whether there is red. The incubation time varies according to different types of bacteria. See the result explanation for details.
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Number of views:
1000
Product serial number
(ID1810)
Specifications:
30 test / box
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Product description
Parameters
β- Lactamase test
Purpose:
Used to detect some bacteria β- Lactamases, such as Staphylococcus aureus, Haemophilus influenzae, Neisseria gonorrhoeae, Moraxella catarrhalis, Enterococcus faecalis and Bacteroides fragilis.
Principle:
The paper contains cefnitrothiophene. When this β The acyl bond in the lactam ring is β- After lactamase hydrolysis, a rapid color reaction occurs, changing from yellow to red, when a bacterium produces enough β Lactamase, apply the bacteria to the paper, and the paper will change from yellow to red.
Cefnitrothiophene has a wide range of sensitivity and specificity. As a commercialized test β- So far, it has not been found that cefnitrothiophene will react with other microbial enzymes.
β- Test method for lactamase
one    Hold the top of the test strip, slightly wet the filter paper in the test area at the end, and then directly dip or scrape the suspicious typical colonies with the filter paper wet at the end;
two    After incubation at 35 ℃± 2 ℃, observe whether there is red. The incubation time varies according to different types of bacteria. See the result explanation for details.
 
1
 
For most bacteria, the positive reaction is usually within 5 minutes, but for some Staphylococcus, the positive reaction can be as long as 1 hour.
Note: only one strain can be tested per piece of paper.
β- Interpretation of lactamase test results
 
Strain result Approximate reaction time Interpretation of results
Staphylococcus aureus positive

1h

The results explained the resistance to penicillin, amino, carboxyl and urea penicillins.
Haemophilus influenzae positive

1min

Resistant to ampicillin and amoxicillin.
Neisseria gonorrhoeae and Moraxella catarrhalis positive

1min

Resistant to penicillin, ampicillin and amoxicillin.
Enterococcus faecalis positive

5min

Resistant to penicillin, amido and urea penicillin.
Anaerobic bacteria positive

30min

It can be used for the identification of Bacteroides fragilis. It can be explained as resistance to penicillin, ampicillin and amoxicillin, as well as resistance to cephalosporins, including cefotaxime and a few cefoxitin.

 

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Keyword:
cefnitrothiophene paper lactamase
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Time of issue:2021-06-28 17:52:44

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