Language
Service
/
/
Plasma coagulase

Plasma coagulase

Plasma coagulase

(Summary description)Test method of enzyme contact test: 1. Pick several single suspicious colonies to be tested with a small wooden stick or bamboo stick (avoid picking blood agar to avoid false positive reaction of peroxidase in blood), and put the wooden stick or bamboo stick into the small test tube of enzyme contact solution (with the handle on)

  • Categories:Technical scheme
  • Author:
  • Origin:
  • Time of issue:2021-06-29 11:26
  • Views:0
Information
1
 
2
 
Test method of contact enzyme test
1. Pick out several single suspicious colonies to be tested with a small wooden stick or bamboo stick (avoid picking blood agar to avoid false positive reaction of peroxidase in blood), and put the wooden stick or bamboo stick into the small test tube of enzyme contact solution (with the handle on);
2. Observe whether a large number of bubbles are generated immediately and violently.
 
3
 
  血浆凝固酶试验检验方法
  1.向一支干粉兔血浆小试管中加入约0.5mL生理盐水使其复溶;
  2.用人造丝拭子(勿用医用棉签)或接种环刮/挑取可疑待测菌落至试管中,洗脱,靠壁挤压拭子头,使其乳化,形成较浓菌悬液,拧上试管帽;
  3.35℃±2℃孵育,至少每小时观察一次直至4h,一旦出现凝固就判为阳性。观察时切勿振摇或颠倒试管,应横置观察,4h不凝固者为阴性;试验应同时做阳性、阴性对照。
 
4
 
Test method for bile aescin test (B-E)
1. Take 1 test bile aescin small test tube and use it for each bacteria to be tested;
2. Unscrew the test tube cap, scrape / pick out the suspected colonies to be tested with a rayon swab (do not use a medical cotton swab) or an inoculation ring into a bile aescin small test tube, elute, press the swab head against the wall to make it emulsified into a concentrated bacterial suspension;
3.35 ℃± 2 ℃, incubation for 1H ~ 2H; Observe whether there is brownish black or black.
 
5
 
Test method for pyrrolidone test (Pyr)
1. Soak the filter paper at the end of Pyr test strip with Pyr buffer a;
2. Use the inoculation ring to pick out the suspicious colony to be tested from the appropriate agar medium, and smear it on the end filter paper or dip it directly;
3. Place it in a closed container (such as an empty plate); 35 ℃± 2 ℃, incubation for 5min ~ 10min;
4. Add a drop of Pyr buffer solution B to the end where bacteria are applied, and observe whether there is red.
 
6
 
Test method for hippurate
1. Take a hippurate test tube for each bacteria to be tested;
2. Scrape / pick out the suspected colonies to be tested with rayon swab (do not use medical cotton swab) or small wooden stick or inoculation ring into the small test tube of sodium hippurate solution (do not pick up agar, because the existence of protein can cause a weak positive reaction), elute, squeeze the swab head against the wall to emulsify it to form a concentrated bacterial suspension, and tighten it;
3.35 ℃± 2 ℃, incubation for 1.5h ~ 2H;
4. Unscrew the test tube cap, add about 0.2ml (about 4 drops in the dropping bottle) of ninhydrin solution, shake it gently to mix it, and screw on the test tube cap;
5.35 ℃± 2 ℃, incubation for 5min ~ 10min; Observe whether there is dark purple blue (false positive results can be produced if the incubation time exceeds 20min).
 
7
Keyword:

Scan the QR code to read on your phone

- More programs -

Oxidase
Oxidase
2021-06-29
Operating steps of oxidase test: 1. Hold the test strip and directly dip or scrape the suspicious typical colony with the test paper area at the end; 2. Observe whether there is dark purple blue within 10s at room temperature.
See more information
Operating steps of oxidase test: 1. Hold the test strip and directly dip or scrape the suspicious typical colony with the test paper area at the end; 2. Observe whether there is dark purple blue within 10s at room temperature.
Plasma coagulase
Plasma coagulase
Test method of enzyme contact test: 1. Pick several single suspicious colonies to be tested with a small wooden stick or bamboo stick (avoid picking blood agar to avoid false positive reaction of peroxidase in blood), and put the wooden stick or bamboo stick into the small test tube of enzyme contact solution (with the handle on)
See more information
Test method of enzyme contact test: 1. Pick several single suspicious colonies to be tested with a small wooden stick or bamboo stick (avoid picking blood agar to avoid false positive reaction of peroxidase in blood), and put the wooden stick or bamboo stick into the small test tube of enzyme contact solution (with the handle on)
Moraxella catarrhalis
Moraxella catarrhalis
Moraxella catarrhalis (MC) was first discovered in 1896. At that time, it was called Micrococcus catarrhalis. Later, it was also called Neisseria catarrhalis and Branhamella catarhalis. Gram negative bacteria are shown by Gram staining. In the past, Moraxella catarrhalis was considered to be a normal parasite of the upper respiratory tract without pathogenicity to human body. However, studies over the past 20 years have found that the bacterium can not only cause upper respiratory tract infection in children and the elderly, but also an important pathogen causing lower respiratory tract infection in adults. It is the third most common pathogen of maxillary sinusitis, otitis media, pneumonia in children and chronic lower respiratory tract infection in adults, second only to Haemophilus influenzae and Streptococcus pneumoniae, Moreover, the incidence rate is increasing year by year, especially in patients with chronic obstructive pulmonary disease. Therefore, rapid identification of MC has important clinical significance. We identified MC by indole acetate disk method.
See more information
Moraxella catarrhalis (MC) was first discovered in 1896. At that time, it was called Micrococcus catarrhalis. Later, it was also called Neisseria catarrhalis and Branhamella catarhalis. Gram negative bacteria are shown by Gram staining. In the past, Moraxella catarrhalis was considered to be a normal parasite of the upper respiratory tract without pathogenicity to human body. However, studies over the past 20 years have found that the bacterium can not only cause upper respiratory tract infection in children and the elderly, but also an important pathogen causing lower respiratory tract infection in adults. It is the third most common pathogen of maxillary sinusitis, otitis media, pneumonia in children and chronic lower respiratory tract infection in adults, second only to Haemophilus influenzae and Streptococcus pneumoniae, Moreover, the incidence rate is increasing year by year, especially in patients with chronic obstructive pulmonary disease. Therefore, rapid identification of MC has important clinical significance. We identified MC by indole acetate disk method.
Bile lysozyme
Bile lysozyme
2021-06-29
Operation steps of bile bacteriolysis test:1 test tube method,1) For the test tube, scrape / pick out the suspicious colony to be tested with rayon swab (do not use medical cotton swab) or inoculation ring into the small test tube of bile salt solution
See more information
Operation steps of bile bacteriolysis test:1 test tube method,1) For the test tube, scrape / pick out the suspicious colony to be tested with rayon swab (do not use medical cotton swab) or inoculation ring into the small test tube of bile salt solution
Porphyrin
Porphyrin
2021-06-29
Test method for porphyrin synthesis test (ALA) 1. Soak the filter paper at the end of ALA test strip with ALA buffer; 2. Use the inoculation ring to pick out the suspected colony to be tested from the chocolate agar medium, and smear it on the end filter paper or dip it directly;
See more information
Test method for porphyrin synthesis test (ALA) 1. Soak the filter paper at the end of ALA test strip with ALA buffer; 2. Use the inoculation ring to pick out the suspected colony to be tested from the chocolate agar medium, and smear it on the end filter paper or dip it directly;
MUG
MUG
2021-06-29
Operation steps of Mug test: 1. Fully soak the filter paper at the end of the mug test strip with mug buffer; 2. Pick out the suspicious colony to be tested with the inoculation ring, smear it on the end filter paper or dip it directly;
See more information
Operation steps of Mug test: 1. Fully soak the filter paper at the end of the mug test strip with mug buffer; 2. Pick out the suspicious colony to be tested with the inoculation ring, smear it on the end filter paper or dip it directly;

底部版权

Time of issue:2021-06-28 17:52:44

TEL:+86-23-88613755  88613756 ADD:Chongqing Jiangbei District No. 48 Road, road, industrial park, No. 2, No.

© 2021 Chongqing Pang Tong Medical Devices Co., Ltd ICP:渝ICP备16005976号-1 Powered By: www.300.cn

Ustar