Operating steps of oxidase test: 1. Hold the test strip and directly dip or scrape the suspicious typical colony with the test paper area at the end; 2. Observe whether there is dark purple blue within 10s at room temperature.
Test method of enzyme contact test: 1. Pick several single suspicious colonies to be tested with a small wooden stick or bamboo stick (avoid picking blood agar to avoid false positive reaction of peroxidase in blood), and put the wooden stick or bamboo stick into the small test tube of enzyme contact solution (with the handle on)
Moraxella catarrhalis (MC) was first discovered in 1896. At that time, it was called Micrococcus catarrhalis. Later, it was also called Neisseria catarrhalis and Branhamella catarhalis. Gram negative bacteria are shown by Gram staining. In the past, Moraxella catarrhalis was considered to be a normal parasite of the upper respiratory tract without pathogenicity to human body. However, studies over the past 20 years have found that the bacterium can not only cause upper respiratory tract infection in children and the elderly, but also an important pathogen causing lower respiratory tract infection in adults. It is the third most common pathogen of maxillary sinusitis, otitis media, pneumonia in children and chronic lower respiratory tract infection in adults, second only to Haemophilus influenzae and Streptococcus pneumoniae, Moreover, the incidence rate is increasing year by year, especially in patients with chronic obstructive pulmonary disease. Therefore, rapid identification of MC has important clinical significance. We identified MC by indole acetate disk method.
Operation steps of bile bacteriolysis test:1 test tube method,1) For the test tube, scrape / pick out the suspicious colony to be tested with rayon swab (do not use medical cotton swab) or inoculation ring into the small test tube of bile salt solution
Test method for porphyrin synthesis test (ALA) 1. Soak the filter paper at the end of ALA test strip with ALA buffer; 2. Use the inoculation ring to pick out the suspected colony to be tested from the chocolate agar medium, and smear it on the end filter paper or dip it directly;