Hippurate

Description 

Recommended in qualitative procedures to detect organisms possessing the enzyme hippurate hydrolase ,  which promotes the hydrolysis of the peptide bond in hippurate, releasing glycine and benzoic acid as end products. The benzoic acid can be detected by using ferric chloride indicator. To detect the glycine and nynhydrin is possible, but any free amino acid will interfere. The tube method is a rapid test used for presumptive identification of Gardnerella vaginalis, Campylobacter jejuni, Listeria monocytogenes and β-hemolytic group B streptococci.

Directions 

Step1 Unfreeze one tube of Hippurate reagent for each colonyunder test

Step2 Pickup the colony under test with a stick or loop into a small with Hippurate reagent

Step3 Incubate at 35 °C in ambient air for 1.5h-2h

Step4 Drop0.2ml ninhydrin into it and shake softly

Step5 incubate at 35 °C for 5-10min and observe

Notice:

1.Check the product appearance before use, or else do quality control and discard

2.Only be used once

3．Store at -20℃，away from light

4.  Use safely.

5.  Get and store immediately.

6.  Put unspent products back to the packaging bag on request.

7.  After use, products should be discarded.