PYR test

The Presence of the enzyme PYR is often tested to distinguish S. pyogenes from other beta-hemolytic streptococci. Hydrolysis of L-pyrrolidony-β-naphthylamide by the enzyme to β-naohthylamine produces a red color with the addition of the chromogenic reagent. It is important to distinguish Group A Streptococcus from Enterococcus prior to PYR test .It can be used together with other test products flexibly.

Directions:

Step1 Wet the test area with Buffer Solution A

Step2 Pick the colony fromthe suitable agar with a loop and smearthem on the test area.

Step3 Incubate at 35 °C for 5-10min in a sealed container

Step4 DropBuffer Solution B on test area which colony was smeared and

      observe whether it becomes redor not.

Notice:

1.Check the product appearance before use, or else do quality control and discard

2.Only be used once

3．Store at 2-8℃，away from light

4.  Use safely.

5.  Get and store immediately.

6.  Put unspent products back to the packaging bag on request.

7.  After use, products should be discarded.