Test method of contact enzyme test
1. Pick out several single suspicious colonies to be tested with a small wooden stick or bamboo stick (avoid picking blood agar to avoid false positive reaction of peroxidase in blood), and put the wooden stick or bamboo stick into the small test tube of enzyme contact solution (with the handle on);
2. Observe whether a large number of bubbles are generated immediately and violently.

 

 

　　血浆凝固酶试验检验方法

　　1.向一支干粉兔血浆小试管中加入约0.5mL生理盐水使其复溶；

　　2.用人造丝拭子（勿用医用棉签）或接种环刮/挑取可疑待测菌落至试管中，洗脱，靠壁挤压拭子头，使其乳化，形成较浓菌悬液，拧上试管帽；

　　3.35℃±2℃孵育，至少每小时观察一次直至4h，一旦出现凝固就判为阳性。观察时切勿振摇或颠倒试管，应横置观察，4h不凝固者为阴性；试验应同时做阳性、阴性对照。

 

 

Test method for bile aescin test (B-E)
1. Take 1 test bile aescin small test tube and use it for each bacteria to be tested;
2. Unscrew the test tube cap, scrape / pick out the suspected colonies to be tested with a rayon swab (do not use a medical cotton swab) or an inoculation ring into a bile aescin small test tube, elute, press the swab head against the wall to make it emulsified into a concentrated bacterial suspension;
3.35 ℃± 2 ℃, incubation for 1H ~ 2H; Observe whether there is brownish black or black.

 

 

Test method for pyrrolidone test (Pyr)
1. Soak the filter paper at the end of Pyr test strip with Pyr buffer a;
2. Use the inoculation ring to pick out the suspicious colony to be tested from the appropriate agar medium, and smear it on the end filter paper or dip it directly;
3. Place it in a closed container (such as an empty plate); 35 ℃± 2 ℃, incubation for 5min ~ 10min;
4. Add a drop of Pyr buffer solution B to the end where bacteria are applied, and observe whether there is red.

 

 

Test method for hippurate
1. Take a hippurate test tube for each bacteria to be tested;
2. Scrape / pick out the suspected colonies to be tested with rayon swab (do not use medical cotton swab) or small wooden stick or inoculation ring into the small test tube of sodium hippurate solution (do not pick up agar, because the existence of protein can cause a weak positive reaction), elute, squeeze the swab head against the wall to emulsify it to form a concentrated bacterial suspension, and tighten it;
3.35 ℃± 2 ℃, incubation for 1.5h ~ 2H;
4. Unscrew the test tube cap, add about 0.2ml (about 4 drops in the dropping bottle) of ninhydrin solution, shake it gently to mix it, and screw on the test tube cap;
5.35 ℃± 2 ℃, incubation for 5min ~ 10min; Observe whether there is dark purple blue (false positive results can be produced if the incubation time exceeds 20min).